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Flowering Plants


Internal Structure of a Leaf







Figure: internal structure of a leaf

When the leaf is cut in cross-section and seen under a microscope, the below structures are seen:
Figure: Leaf anatomy

  •      Cuticle:

Cuticle is a transparent waxy layer covered on the upper surface of the leaf
Cuticle is made up of wax which is secreted by the epidermal cells
Functions of the cuticle:
a)    Cuticle allows light to pass through
b)    Cuticle reduces water loss (acts as a waterproofing of the leaf)

  •       Epidermis

Epidermis of the leaf consists of a single layer of cells surrounding the whole leaf.
The epidermal cells of the leaf don’t contain chloroplast.
Functions of the epidermis:
a)    Acts as a protective layer
b)    Keeps the leaf’s shape

  Epidermis of the leaf is divided into
A.   Upper epidermis
-       Upper epidermis is found on the upper surface of the leaf and can be seen when the leaf is cut in cross-section and observed under microscope.
-       Cells of the upper epidermis are transparent
-       No stomata are present
Functions of the upper epidermis:
a)    Allow light to pass through
b)    Act as a barrier to micro-organisms
c)    Secrete wax
B.   Lower epidermis
-       Lower epidermis is found on the lower surface of the leaf and can be seen when the leaf is cut in cross-section and observed under microscope.
-       Stomata are present
Functions of the lower epidermis:
a)    Act as a protective layer
b)    It is the site of gaseous exchange into and out of the leaf as stomata are present
  •       Mesophyll tissue

Mesophyll is the tissue between upper and lower epidermis of the leaf
Mesophyll tissue consists of:
a)    Palisade cells (palisade mesophyll cells) ad
b)    Spongy cells (spongy mesophyll cells)
Palisade Mesophyll:
-       Palisade cells are elongated cells and box-like shape
-       Palisade cells are found below the upper epidermis
-       Palisade cells are packed together
-       Palisade cells contain maximum amount of the leaf’s chloroplasts
-       Palisade cells are the main region for photosynthesis in the leaf
-       Palisade cells receive carbon dioxide by the diffusion from air spaces in the spongy mesophyll.
-       Chloroplasts in the palisade cells are able to move within the cytoplasm. During dim light, chloroplasts in the palisade cells move to the upper parts of the cell allowing them maximum absorption of light. In bright light, chloroplasts in the palisade cells move to the lower parts of the cell for protection from the bleaching effects of intense light.
Function of the palisade cells:
                                              i.        Palisade cells are the photosynthetic site in the leaf
Spongy Mesophyll:
-       Spongy cells are spherical and irregularly shaped cells
-       Spongy cells are loosely packed together
-       The air spaces between spongy cells are known as intercellular air spaces.
-       Air spaces between spongy cells allow gaseous exchange in the leaf.
-       Spongy cells allow of gaseous exchange in the leaf.
-       Spongy cells contain viewer chloroplasts
Function of the spongy cells:
i.      Spongy cells are site of gaseous exchange in the leaf.
  •       Vascular bundles

This is the leaf vein, made up of both xylem and phloem.
Xylem vessels bring water and minerals to the leaf cells.
Phloem vessels transport sugars and amino acids away from the leaf cells (this is called translocation).
They also provide support for the leaf
  •      Stomata

Stomata are tiny pores found at the lower epidermis or underside of the leaf.
Stomata are always open during day time and close in the night time.
Each stoma is surrounded by a pair of guard cells.
Guard cells control whether the stoma is open or closed.
Carbon dioxide diffuses in and oxygen diffuses out of the stomata during photosynthesis.
Water vapour passes out of the stomata during transpiration.
Functions of the Stomata:
                      I.        Allow gaseous exchange
                    II.        Transpiration takes place
Function of the guard cells:
                      I.        Control whether the stoma is open or closed


Figure: Open and closed stoma




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